期刊
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
卷 148, 期 -, 页码 68-73出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2018.03.016
关键词
Rhyzopertha dominica; Spinosad; Nicotinic acetylcholine receptor subunit alpha 6; Mutation; Transcriptional expression
资金
- Ministry of Science and Technology, Taiwan [104-2313-B-005-017-]
The lesser grain borer, Rhyzopertha dominica, which is a primary pest of stored products, breaks up whole grains and makes them susceptible to secondary infestation by other pests. Insecticide application is the main control measure against this borer. A resistant strain of R. dominica against the insecticide, spinosad, was selected in the laboratory. The full-length cDNA of the target site of spinosad, nicotinic acetylcholine receptor subunit alpha 6, from R. dominica (Rda6) was cloned and analyzed using reverse transcription PCR and rapid amplification of eDNA ends. The complete 2133-bp eDNA contains the open reading frame of 1497 by encoding a 498-amino-acid protein. There are four predicted transmembrane (TM) regions, and six extracellular ligand-binding sites at the N-terminus, upstream from the first TM in Rd alpha 6. Three mutations have been found in the resistant strain compared with the susceptible one: (1) a 181-bp fragment truncated at the N-terminus, resulting in the appearance of a premature stop codon, (2) one missing bp at the position 997, causing a frame-shift mutation, and (3) an 87-bp fragment truncated in the TM2 region. In addition, real-time quantitative PCR was applied to detect the transcriptional expression of Rd alpha 6 in both the susceptible and resistant strains. The results indicated that the expression of Rd alpha 6 was significantly lower in then resistant strain than in susceptible one. In conclusion, mutation of Rd alpha 6 may cause R. dominica resistant to spinosad due to target site insensitivity.
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