期刊
PEST MANAGEMENT SCIENCE
卷 75, 期 1, 页码 18-28出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/ps.5126
关键词
dsRNA stability; dsRNases; endocytosis; target gene; exogenous RNAi; systemic RNAi
资金
- National Natural Science Foundation of China [NSFC31730074]
- U.S. Department of Agriculture [USDA/NIFA 2017-09394, USDA/NIFA 2014-67013-21714]
- Kansas Agricultural Experiment Station [KS471]
RNA interference (RNAi) is an endogenous, sequence-specific gene-silencing mechanism elicited by small RNA molecules. RNAi is a powerful reverse genetic tool, and is currently being utilized for managing insects and viruses. Widespread implementation of RNAi-based pest management strategies is currently hindered by inefficient and highly variable results when different insect species, strains, developmental stages, tissues, and genes are targeted. Mechanistic studies have shown that double-stranded ribonucleases (dsRNases), endosomal entrapment, deficient function of the core machinery, and inadequate immune stimulation contribute to limited RNAi efficiency. However, a comprehensive understanding of the molecular mechanisms limiting RNAi efficiency remains elusive. Recent advances in dsRNA stability in physiological tissues, dsRNA internalization into cells, the composition and function of the core RNAi machinery, as well as small-interfering RNA/double-stranded RNA amplification and spreading mechanisms are reviewed to establish a global understanding of the obstacles impeding wider understanding of RNAi mechanisms in insects. (c) 2018 Society of Chemical Industry
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