期刊
INTERNATIONAL JOURNAL OF ORAL SCIENCE
卷 5, 期 1, 页码 21-25出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ijos.2013.15
关键词
dental plaque; head-and-neck radiotherapy; microbial diversity; oral microbiota; pyrosequencing
资金
- National Natural Science Foundation of China [81070826/30872886]
- Shanghai Rising-Star Program [12QH1401400]
- Shanghai Jiao Tong University [YG2011MS67]
The objective of this study was to investigate the compositional profiles and microbial shifts of oral microbiota during head-and-neck radiotherapy. Bioinformatic analysis based on 16S rRNA gene pyrosequencing was performed to assess the diversity and variation of oral microbiota of irradiated patients. Eight patients with head and neck cancers were involved in this study. For each patient, supragingival plaque samples were collected at seven time points before and during radiotherapy. A total of 147 232 qualified sequences were obtained through pyrosequencing and bioinformatic analysis, representing 3 460 species level operational taxonomic units (OTUs) and 140 genus level taxa. Temporal variations were observed across different time points and supported by cluster analysis based on weighted UniFrac metrics. Moreover, the low evenness of oral microbial communities in relative abundance was revealed by Lorenz curves. This study contributed to a better understanding of the detailed characterization of oral bacterial diversity of irradiated patients. International Journal of Oral Science (2013) 5, 21-25; doi:10.1038/ijos.2013.15; published online 29 March 2013
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