期刊
MASS SPECTROMETRY LETTERS
卷 4, 期 2, 页码 38-40出版社
KOREAN SOC MASS SPECTROMETRY
DOI: 10.5478/MSL.2013.4.1.38
关键词
Glycans; MALDI; PNGase F; ovalbumin; oligosaccharides; dihydroxybenzoic acid
类别
资金
- National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [2010-0010776]
Glycans released from ovalbumin by PNGase F were analyzed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry using three different dihydroxybenzoic acid (DHB) matrix systems: 2,5-DHB, 2,6-DHB, and a 2,5-DHB/2,6-DHB binary matrix. Relative to the results obtained with the single-component matrices (2,5-DHB or 2,6-DHB), the 2,5-DHB/2,6-DHB binary matrix boasted lower background noise and higher sensitivity. A total of 16 glycan peaks were observed using the 2,5-DHB/2,6-DHB binary matrix, while only 10 and 9 glycan peaks were observed using the 2,5-DHB and 2,6-DHB matrices, respectively.
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