期刊
PARASITOLOGY RESEARCH
卷 117, 期 8, 页码 2555-2567出版社
SPRINGER
DOI: 10.1007/s00436-018-5944-1
关键词
Giardia lamblia; Mast cells; Activating molecules; Arginine deiminase
类别
资金
- Fondo de Investigacion en Salud, Instituto Mexicano del Seguro Social (IMSS) [FIS/IMSS/PROT/G12/1128, FIS/IMSS/G13/1233]
- CONACYT [CB 2007/80647]
- IMSS
- Fundacion IMSS
Mast cells play a central role in the early clearance of the intestinal parasite Giardia lamblia. In a previous study, we reported that G. lamblia live trophozoites or trophozoite-derived total soluble extract induced direct activation (IgE-independent) of mast cells and release of IL-6 and TNF-alpha. To identify the Giardia molecules and the mast cell receptors involved in this activation, trophozoite-derived total soluble proteins separated into three fractions (F1-F3) were evaluated for its ability to activate mast cells in vitro. F2 activated mast cells in a greater extent than F1 and F3. Furthermore, F2 induced the release of IL-6 and TNF-alpha by mast cells. TLR2 and TLR4 expression increased slightly after mast cell stimulation with either F2 or total soluble extract; however, these receptors were not involved in F2 or total soluble extract-induced proinflammatory cytokine production. Proteins present in F2 as unique and high-intensity bands identified by liquid chromatography coupled with tandem mass spectrometry, include molecules with important biological activities such as enolase and arginine deiminase (ADI). Recombinant ADI and enolase were tested for their ability to activate mast cells, but only ADI induced a significant release of IL-6 and TNF-alpha. ADI product, citrulline but not ammonium, also induced mast cell release of TNF-alpha. Interestingly, recombinant ADI still stimulated the secretion of TNF-alpha by mast cells in a arginine-free medium, although in a lower extend that in the presence of arginine, indicating that either ADI itself can stimulate mast cells or through its metabolic product, citrulline.
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