期刊
PANCREATOLOGY
卷 18, 期 6, 页码 661-665出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.pan.2018.06.002
关键词
Biomarkers for pancreatic ductal adenocarcinoma; Nanotechnology; Pancreatic adenocarcinoma clinical stage; Protein corona; Pancreatic ductal adenocarcinoma
资金
- Italian Minister of Health [GR-2011-02350094]
- AIRC (Italian Association for Cancer Research) [IG 2017 Id.20327]
Background: Pancreatic ductal adenocarcinoma (PDAC) early diagnosis is crucial and new, cheap and user-friendly techniques for biomarker identification are needed. Protein corona (PC) is emerging a new bio-interface potentially useful in tumor early diagnosis. In a previous investigation, we showed that relevant differences between the protein patterns of PCs formed on lipid NPs after exposure to PDAC and non-cancer plasma samples exist. To extend that research, We performed this pilot study to investigate the effect of PDAC tumor size and distant metastases on PC composition. Methods: Twenty PDACs were clinically staged according to the UICC TNM staging system 8th Edition. Collected plasma samples were let to interact with lipid NPs; resulting PCs were characterized by SDSPAGE. To properly evaluate changes in the PC, the protein intensity profiles were reduced to four regions of molecular weight: < 25 kDa, 25-50 kDa, 50-120 kDa, > 120 kDa. Results: Data analysis allowed to distinguish T1 -T2 cases from T3 and above all from metastatic ones (p <0.05). Discrimination power was particularly due to a subset of plasma proteins with molecular weight comprised between 25-50 kDa and 50-120 kDa. Conclusions: PC composition is critically influenced by tumor size and presence of distant metastases in PDAC. If our findings will be further confirmed, we envision that future developments of cheap and userfriendly PC-based tools will allow to improve the accuracy of PDAC clinical staging, identifying among resectable PDACs with potentially better prognosis (i.e. T1 and T2) those at higher risk of occult distant metastases. (C) 2018 IAP and EPC. Published by Elsevier B.V. All rights reserved.
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