期刊
HYDROBIOLOGIA
卷 760, 期 1, 页码 81-89出版社
SPRINGER
DOI: 10.1007/s10750-015-2304-3
关键词
Chlorella vulgaris; Chlorella thermophila; Microalgae; Invading species; Rooftop incubator; Phylogenetic analysis
资金
- Hainan Provincial Science and Technology Key Program [GCZX2013001, ZDZX2013023]
- International Science and Technology Cooperation Program of China [2014DFA30680]
- Natural Science Foundation of Hainan Province [309051]
Open rooftop incubators may provide a cost-effective way for producing microalgal biomass for biodiesel production without requiring large land areas. While cultivating a Chlorella vulgaris strain with high oil content in a rooftop incubator, we identified an invading thermo-resistant green alga, which replaced the original culture. The isolated strain HTA1-65 had the typical Chlorella morphology with spherical cells and a cup-shaped parietal chloroplast. The protoplast contained a single pyrenoid traversed by a double-layered thylakoid and enveloped by a single starch sheath. The oil content of this strain was only 21.5 %, one third of the original strain. This strain grew at 42 A degrees C, with an optimum of 33 A degrees C. Heat treatment of 45 A degrees C for 3 h per day did not decrease its growth rate, suggesting that it has a high potential in biotechnological applications. Phylogenetic analyses revealed that HTA1-65 belongs to the genus Chlorella, but a specific relationship to any species was not indicated by nuclear and chloroplast small subunit rRNA genes. However, based on at least one compensating base change (CBC) and a few hemi-CBCs in the ITS2 secondary structure of closely related species, we describe strain HTA1-65 as a novel, thermo-tolerant Chlorella species, C. thermophila.
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