期刊
OPTICS LETTERS
卷 43, 期 7, 页码 1423-1426出版社
OPTICAL SOC AMER
DOI: 10.1364/OL.43.001423
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资金
- National Basic Research Program of China [2015CB352003]
- National Natural Science Foundation of China (NSFC) [61335003, 61427818, 61735017]
- National Key Research and Development Program of China [2016YFF0101400, 2017YFC0110303]
- NSFC of Zhejiang province [LR16F050001]
- Fundamental Research Funds for the Central Universities [2017FZA5004]
Measuring three-dimensional nanoscale cellular structures is challenging, especially when the structure is dynamic. Owing to the informative total internal reflection fluorescence (TIRF) imaging under varied illumination angles, multi-angle (MA) TIRF has been examined to offer a nanoscale axial and a subsecond temporal resolution. However, conventional MA-TIRF still performs badly in lateral resolution and fails to characterize the depth image in densely distributed regions. Here, we emphasize the lateral super-resolution in the MA-TIRF, exampled by simply introducing polarization modulation into the illumination procedure. Equipped with a sparsity and accelerated proximal algorithm, we examine a more precise 3D sample structure compared with previous methods, enabling live cell imaging with a temporal resolution of 2 s and recovering high-resolution mitochondria fission and fusion processes. We also shared the recovery program, which is the first open-source recovery code for MA-TIRF, to the best of our knowledge. (c) 2018 Optical Society of America
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