期刊
HUMAN IMMUNOLOGY
卷 76, 期 5, 页码 329-338出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.humimm.2015.03.007
关键词
Cord blood mononuclear cell; Chorioamnionitis; Bronchopulmonary dysplasia; CD4(+) T cells; IL-6
类别
资金
- Department of Pediatrics at University of Rochester Medical Center, NY, USA
- National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services [HHSN27220120005C]
- [NIHT32HD057821]
- [NHLBIU01HL101813]
Background: Chorioamnionitis (CA) is associated with premature delivery and bronchopulmonary dysplasia (BPD). We hypothesize that preterm infants exposed to CA have reduced suppressive regulatory T cells (Treg) and increased non-regulatory T cell pro-inflammatory cytokines, increasing risk for BPD. Objective: To evaluate cord blood CD,er T cell regulatory phenotype and pro-inflammatory cytokine production in CA and BPD groups. Study design: Cord blood mononuclear cells from infants (GA <= 32 weeks), with or without placental histological evidence of CA (hChorio), were analyzed by flow cytometry. Clinical information was collected by retrospective chart review. Numbers of putative Treg (CD4(+)FoxP3(+)CD25(+)CD127Dim), CD4(+) non-Tregs, and CD4(+) T cell intracellular cytokine content following in vitro stimulation were compared with CA status and oxygen requirement at 36 weeks postmenstrual age. Result: Absolute Treg numbers were not different in CA and non-CA exposed samples. However, the infants who developed BPD had a significant decrease in Treg and non-regulatory T cell numbers. Greater IL-6 production was observed in hCA group. Conclusion: A pro-inflammatory CD4(+) T cell status is noted in CA and BPD but the later disease is also associated with decrease in Tregs, suggesting that the development of BPD is marked by distinct inflammatory changes from those of CA exposed infants. (C) 2015 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.
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