期刊
HUMAN IMMUNOLOGY
卷 76, 期 5, 页码 307-317出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.humimm.2015.03.010
关键词
CCR7; Dendritic cells; Maturation; MicroRNA; Promoter
类别
资金
- Lebanese National Council for Scientific Research (CNRS)
- Lebanese University
- Chaieb Foundation
- Belgian Fonds National de la Recherche Scientifique (FRSM, Televie)
- MEDIC Foundation
- International Brachet Stiftung
- Lambeau-Marteaux Foundation
- lea Amis de l'Institut Bordet
- Van Buuren Foundation
- Hoguet Foundation
The chemokine C receptor 7 (CCR7) is a G-protein-coupled heptahelical receptor (GPCR) that is expressed on a wide variety of cells including memory T cells, B cells, mature dendritic cells, and cancer cells. Activated by its ligands CCL19 or CCL21, CCR7 plays a major role in metastasis of cancer cells. Recent studies demonstrated the role of NF-kappa B and AP-1 transcription factors in addition to let-7 microRNA in CCR7 expression. Our ChIP assays further show the binding of Sp-1, Sp-3 and NFAT-1 transcription factors to their potential binding sites in the 1Kb promoter region with the later found to inhibit whilst Sp-1, and Sp-3 were found to stimulate CCR7 expression as demonstrated by transfection assays. On the other hand, in addition to the known let-7 regulation of CCR7, we found miR-21 to have a highly conserved target region in CCR7 3'UTR and to be significantly down-regulated during the course of dendritic cell maturation, allowing for high expression of CCR7. (C) 2015 Published by Elsevier Inc. on behalf of American Society for Histocompatibility and Immunogenetics.
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