期刊
JOURNAL OF APICULTURAL SCIENCE
卷 57, 期 1, 页码 25-32出版社
RESEARCH INST POMOLOGY FLORICULTURE, DIV APICULTURE
DOI: 10.2478/jas-2013-0003
关键词
oxytetracycline; honey; residues; validation; depletion; HPLC-UV method
类别
A liquid chromatography method with IN detection for determination of oxytetracycline (OTC) in honey has been developed. The samples were extracted with the solution of oxalic acid. The clean-up procedure was performed by solid phase extraction (SPE) using polymeric Strata X and carboxylic acid cartridges. Chromatographic separation was carried out on the Luna C8 analytical column with mobile phase consisting of acetonitrile-0.02 M oxalic acid. The method has been successfully validated according to the requirements of the European Decision 2002/6571EC and this method is used in routine control of oxytetracycline in honey samples. The limit of detection (LOD) and limit of quantification (LOQ) of the presented method were 10 and 12.5 mu g/kg, respectively. The developed method has also been verified in quantitative determination of oxytetracycline residues in honey after experimental treatment with this product in bee colonies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据