期刊
NUCLEIC ACIDS RESEARCH
卷 46, 期 10, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gky164
关键词
-
资金
- Netherlands Organization for Scientific Research ZonMW-TOP [91210033, 91211061]
- European Research Council Advanced Grant [694466]
- Danish Council for independent Research, Medical Sciences [DFF-4183-00599]
- Danish Cancer Society [R91-A7351-14-S9]
- European Research Council [AdG 694466]
Template-directed CRISPR/Cas9 editing is a powerful tool for introducing subtle mutations in genomes. However, the success rate of incorporation of the desired mutations at the target site is difficult to predict and therefore must be empirically determined. Here, we adapted the widely used TIDE method for quantification of templated editing events, including point mutations. The resulting TIDER method is a rapid, cheap and accessible tool for testing and optimization of template-directed genome editing strategies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据