期刊
NUCLEIC ACIDS RESEARCH
卷 46, 期 8, 页码 4129-4137出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gky200
关键词
-
资金
- National Research Foundation of Korea [NRF-2014R1A1A2055681, NRF-2017R1A2B4010632, NRF-2016R1A2B4014855]
- Institute for Basic Science [IBS-R023-D1]
Left-handed Z-DNA is an extraordinary conformation of DNA, which can form by special sequences under specific biological, chemical or physical conditions. Human ADAR1, prototypic Z-DNA binding protein (ZBP), binds to Z-DNA with high affinity. Utilizing single-molecule FRET assays for Z-DNA forming sequences embedded in a long inactive DNA, we measure thermodynamic populations of ADAR1-bound DNA conformations in both GC and TG repeat sequences. Based on a statistical physics model, we determined quantitatively the affinities of ADAR1 to both Z-form and B-form of these sequences. We also reported what pathways it takes to induce the B-Z transition in those sequences. Due to the high junction energy, an intermediate B* state has to accumulate prior to the B-Z transition. Our study showing the stable B* state supports the active picture for the protein-induced B-Z transition that occurs under a physiological setting.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据