期刊
NUCLEIC ACIDS RESEARCH
卷 46, 期 13, 页码 6823-6840出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gky324
关键词
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资金
- Biotechnology and Biological Sciences Research Council [BB/F017154/1]
- University of Nottingham international PhD scholarship
- Apoio a formacion posdoutoral do PLAN I2C da Xunta de Galicia
- Engineering and Physical Sciences Research Council [EP/N006615/1]
- University of Malaya High Impact Research [H-50001-A000027]
- Research Councils of the United Kingdom: Biotechnology and Biological Sciences Research Council & Engineering and Physical Sciences Research Council
- [PG085-2015B]
- BBSRC [BB/R012415/1] Funding Source: UKRI
- EPSRC [EP/N006615/1] Funding Source: UKRI
Pseudomonads typically carrymultiple non-identical alleles of the post-transcriptional regulator rsmA. In Pseudomonas aeruginosa, RsmN is notable in that its structural rearrangement confers distinct and overlapping functions with RsmA. However, little is known about the specificities of RsmN for its target RNAs and overall impact on the biology of this pathogen. We purified and mapped 503 transcripts directly bound by RsmN in P. aeruginosa. About 200 of the mRNAs identified encode proteins of demonstrated function including some determining acute and chronic virulence traits. For example, RsmN reduces biofilm development both directly and indirectly via multiple pathways, involving control of Pel exopolysaccharide biosynthesis and c-di-GMP levels. The RsmN targets identified are also shared with RsmA, although deletion of rsmN generally results in less pronounced phenotypes than those observed for Delta rsmA or Delta rsmArsmN(ind) mutants, probably as a consequence of different binding affinities. Targets newly identified for the Rsm system include the small non-coding RNA CrcZ involved in carbon catabolite repression, for which differential binding of RsmN and RsmA to specific CrcZ regions is demonstrated. The results presented here provide new insights into the intricacy of riboregulatory networks involving multiple but distinct RsmA homologues.
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