4.6 Article

Estimating heat tolerance of plants by ion leakage: a new method based on gradual heating

期刊

NEW PHYTOLOGIST
卷 218, 期 3, 页码 1278-1287

出版社

WILEY
DOI: 10.1111/nph.15097

关键词

acquired heat tolerance; basal heat tolerance; Chl fluorescence; heat acclimation; heat stress; ion leakage; membrane thermostability

资金

  1. Grant Agency of Czech Republic [13-28093S]
  2. Technological Agency of Czech Republic [TA01010254]
  3. Ministry of Education, Youth and Sports of Czech Republic from National Program of Sustainability I [LO1204, LO1415]
  4. Ministry of Education, Youth and Sports of Czech Republic [CZ.02.1.01/0.0/0.0/16_013/0001609]

向作者/读者索取更多资源

Heat tolerance of plants related to cell membrane thermostability is commonly estimated via the measurement of ion leakage from plant segments after defined heat treatment. To compare heat tolerance of various plants, it is crucial to select suitable heating conditions. This selection is time-consuming and optimizing the conditions for all investigated plants may even be impossible. Another problem of the method is its tendency to overestimate basal heat tolerance. Here we present an improved ion leakage method, which does not suffer from these drawbacks. It is based on gradual heating of plant segments in a water bath or algal suspensions from room temperature up to 70-75 degrees C. The electrical conductivity of the bath/suspension, which is measured continuously during heating, abruptly increases at a certain temperature T-COND (within 55-70 degrees C). The T-COND value can be taken as a measure of cell membrane thermostability, representing the heat tolerance of plants/organisms. Higher T-COND corresponds to higher heat tolerance (basal or acquired) connected to higher thermostability of the cell membrane, as evidenced by the common ion leakage method. The new method also enables determination of the thermostability of photochemical reactions in photosynthetic samples via the simultaneous measurement of Chl fluorescence.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据