期刊
NEW JOURNAL OF CHEMISTRY
卷 42, 期 16, 页码 13558-13564出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8nj02038h
关键词
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资金
- National Natural Science Foundation of China [21505011]
- Scientific Research Fund of the Sichuan Provincial Science and Technology Department [2015JY0033]
- Scientific Research Fund of the Sichuan Provincial Education Department [16ZA0356, 18ZA0414]
- Key Laboratory of Green Chemistry of Sichuan Institutes of Higher Education [LYJ1802]
An ultrasensitive sandwich-type electrochemiluminescent (ECL) immunosensor suitable for identifying carcinoembryonic antigens (CEAs) was developed, using a metal-organic framework nanomaterial (AuNP@NPCGO) as an ideal loading platform and Ru(phen)(3)(2+)-doped silica nanoparticles (RuSiNPs) as ECL signaling units. The AuNP@NPCGO nanocomposites were synthesized via in situ deposition of AuNPs on NPCGO as this configuration exhibits high conductivity, strong fixed ability, and excellent stability. The use of RuSiNPs as signaling units provided numerous luminophores that enhanced the ECL signals. Besides the aminated RuSiNPs, which had a large specific surface area and good biocompatibility, functioned as a superior matrix for immobilizing numerous secondary antibodies (Ab(2)). The proposed ECL immunosensor demonstrated excellent performance for CEA detection at concentrations of 0.01 ng mL(-1) to 80 ng mL(-1) with a detection limit of 0.003 ng mL(-1) at a signal-to-noise ratio of three.
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