4.6 Article

Sunitinib Inhibits Inflammatory Corneal Lymphangiogenesis

期刊

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
卷 54, 期 5, 页码 3082-3093

出版社

ASSOC RESEARCH VISION OPHTHALMOLOGY INC
DOI: 10.1167/iovs.12-10856

关键词

corneal neovascularization; sunitinib; lymphangiogenesis

资金

  1. Fonds de la Recherche Scientifique Medicale
  2. Fonds National de la Recherche Scientifique (FNRS, Belgium)
  3. Fondation contre le Cancer
  4. Fonds speciaux de la Recherche (University of Liege)
  5. Plan Cancer'' (Belgium)
  6. Centre Anticancereux pres l'Universite de Liege
  7. Fonds Leon Fredericq (University of Liege)
  8. Action de Recherche Concertee (ULg)
  9. Interuniversity Attraction Poles Programme-Belgian Science Policy (Brussels, Belgium)
  10. three Televie-FNRS grants

向作者/读者索取更多资源

PURPOSE. To evaluate the antilymphangiogenic potential of multi-target tyrosine kinase inhibitor sunitinib in corneal neovascularization (NV). METHODS. Inflammatory corneal NV was induced by thermal cauterization applied in the central cornea of mice, to which sunitinib malate was daily administered by gavage or not. At days 6, 11, or 17 post cauterization, lymphatic and blood vessels, as well as inflammatory cells were immunostained and quantified in whole-mounted corneas. RT-PCRs were performed to evidence VEGF-A, VEGF-C, VEGF-D, placental growth factor (PlGF), and soluble vascular endothelial growth factor receptor (VEGFR)-1 and -2 (sVEGFR-1, sVEGFR-2) expressions. Macrophages were isolated from mice peritoneal cavity following thioglycollate injection to produce conditioned medium. The effects of sunitinib were evaluated in vitro in the aortic and lymphatic ring assays in the presence or not of macrophage conditioned medium. RESULTS. Sunitinib treatment drastically reduced pathologic corneal lymphangiogenesis and angiogenesis. Reduced F4/80+ cell infiltration was evidenced in sunitinib-treated mice and was associated to decreased VEGF-A (by 50%, P < 0.01) and VEGF-C (by 35%, P < 0.01) expressions, while VEGF-D and sVEGFR-2 expressions were not affected. In vitro, sunitinib dose-dependently inhibited aortic ring outgrowth, but failed to affect lymphangiogenesis in the lymphatic ring assay. However, macrophage conditioned medium-enhanced angiogenesis and lymphangiogenesis were both strongly counteracted by sunitinib treatment. Mechanistically, sunitinib blocked VEGFR-2 phosphorylation induced by VEGF-A released by macrophages. CONCLUSIONS. Sunitinib exerts antihemangiogenic and antilymphangiogenic effects in vivo by reducing F4/80+ cell recruitment and interacting with their released factors.

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