A boronate-decorated porous carbon material derived from a zinc-based metal-organic framework for enrichment of cis-diol-containing nucleosides

In this study, a new boronate affinity porous carbon absorbent derived from a home-made zinc-based metal-organic framework (Zn-MOF) was successfully fabricated. This absorbent was prepared by calcination of the Zn-MOF material and further modified with boronic acid groups. The resulting material was used to selectively enrich cis-diol-containing nucleosides in complex samples, and it exhibited superior binding capacity (26.0 mg g(-1) for adenosine). The material coupled with high-performance liquid chromatography was successfully applied to analyze four cis-diol nucleosides (cytidine, uridine, guanosine, and adenosine) in injection and cell samples. Under optimized conditions, the method exhibited excellent extraction performance. The limits of detection of the method were 4.73-14.6 mg L-1 for the four nucleosides based on the signal-to-noise ratio of 3 : 1. Spiked recoveries were found between 88.2 and 108.2% (the relative standard deviation was in range of 1.3-11.3%, n = 3). This study improved the selectivity of porous carbon derived from MOF material and presented a promising thought for effective capturing of analytes on MOF materials.