期刊
NEUROSCIENCE LETTERS
卷 662, 期 -, 页码 389-394出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2017.10.061
关键词
Exoribonuclease; DIS3L2; XRN1; Intranuclear inclusion body disease; Polyglutamine disease
资金
- JSPS KAKENHI [17K07088, 17K07089, 16K19503, 16K15473]
- Hirosaki University Institutional Research Grant
- The Collaborative Research Project of the Brain Research Institute, Niigata University [2017-2810]
- Grants-in-Aid for Scientific Research [17K07088, 16K19503, 16K15473] Funding Source: KAKEN
mRNA turnover controls gene expression under various conditions in aging and neurodegenerative diseases. Polyglutamine (polyQ) diseases and intranuclear inclusion body disease (INIBD) are neurodegenerative diseases characterized by the formation of nuclear inclusions. These inclusions are immunopositive for several proteins associated with amyotrophic lateral sclerosis. In amyotrophic lateral sclerosis, the processing of RNA from gene transcription through degradation (RNA metabolism) has been reported to be altered. We hypothesized that the proteins associated with RNA metabolism are also involved in the formation of nuclear inclusions in polyQ diseases and INIBD. exoribonuclease DIS3L2 and 5'-3' exoribonuclease XRN1 play critical roles in mRNA decay. Using immunohistochemistry with antibodies against DIS3L2 and XRN1, we examined the brains of patients with polyQ diseases and INIBD and normal control subjects. In controls, immunoreactivity for DIS3L2 and XRN1 was found in the neuronal cytoplasm and neuropil, respectively. In INIBD, immunoreactivity for DIS3L2 and XRN1 was present in neuronal and glial nuclear inclusions. Co-localization of ubiquitin and DIS3L2 or XRN1 was demonstrated in these inclusions. In polyQ diseases, however, nuclear inclusions were immunonegative for DIS3L2 and XRN1. These findings suggest that sequestration of exoribonucleases to nuclear inclusions may be related to the pathogenesis of INIBD.
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