期刊
NEUROSCIENCE
卷 379, 期 -, 页码 167-176出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2018.03.016
关键词
Alzheimer's disease; dementia; exosomes; biomarker; microRNA
资金
- Natural Science Foundation of China [81402165/81672913]
- Six talent peaks project in Jiangsu Province [2016-WSW-125]
- 333 Project research projects in Jiangsu Province [BRA2016141]
- Natural Science Foundation of Jiangsu Province [BK20141288/BK20171311]
- Jiangsu Provincial Medical Youth Talent [QNRC2016460/FRC201788]
The aims of this study were to examine the levels of serum and exosomal miR-137, miR-155 and miR-223, three neuroinflammation-related miRNAs, in dementia patients and to explore the value of these miRNAs for the diagnosis and prognostic evaluation of dementia. Thirty-two patients with dementia were enrolled, and sixteen volunteers without dementia served as controls. Serum exosomes were isolated by precipitation with Exo-Quick and characterized by western blotting, nanoparticle-tracking analysis and immunofluorescence microscopy. The levels of both total serum miRNAs and serum exosomal miRNAs were determined by real-time quantitative PCR. Total serum miRNAs and serum exosomal miRNAs were both detected to be downregulated. The median level of serum exosomal miR-223 was significantly decreased in dementia patients (p < 0.01). The level of miR-223 was significantly correlated with Mini-Mental State Examination (MMSE) scores, Clinical Dementia Rating (CDR) scores, magnetic resonance spectroscopy (MRS) spectral ratios and serum concentrations of IL-1 beta, IL-6, TNF-alpha, and CRP. The diagnostic utility of exosomal miR-233 was evaluated by the area under the receiver operating characteristic (ROC) curve, and the area under the curve (AUC) was 0.875. This study suggests that serum exosomal miR-223 is a promising biomarker for diagnosing dementia and evaluating the progression of disease. (C) 2018 IBRO. Published by Elsevier Ltd. All rights reserved.
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