期刊
NEUROREPORT
卷 29, 期 2, 页码 65-70出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/WNR.0000000000000876
关键词
Akt; ATP; NAD(+); NADH; SIRT2
资金
- Major Research Grant from the Scientific Committee of Shanghai Municipality [16JC1400502]
- Chinese National Natural Science Foundation [81271305]
- Shanghai Jiao Tong University Grant for Interdisciplinary Research on Medicine and Engineering
NAD(+) replenishment can restore ATP levels and rescue premature aging in Cockayne syndrome mice. However, there has been no mechanistic study regarding the effects of NAD(+) and NADH on intracellular ATP levels under basal conditions. In our current study, we used BV2 microglia to test our hypothesis that NAD(+) and NADH can increase intracellular ATP levels under basal conditions. We found that both NAD(+) and NADH significantly increased the intracellular ATP levels of BV2 microglia, which were attenuated by SIRT2 siRNA, the SIRT2 inhibitor AGK2, and the phosphatidylinositol 3-kinase/Akt inhibitor LY294002. Our study has also suggested that SIRT2 mediates the NAD(+)-induced and NADH-induced increase in Akt phosphorylation in BV2 microglia. Collectively, our study has suggested that SIRT2 mediates both NAD(+)-induced and NADH-induced increases in the intracellular ATP levels of BV2 microglia by modulating Akt phosphorylation.
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