期刊
NEUROPATHOLOGY
卷 38, 期 4, 页码 337-346出版社
WILEY
DOI: 10.1111/neup.12469
关键词
blood-brain barrier; caveolin-1; caveolin-2; cerebral ischemia reperfusion; transient receptor potential vanilloid 4
资金
- Science and Technology Plan Project of Educational Department of Liaoning Province [L2015535]
- Science and Research Foundation for College Student of Shenyang Medical College [20160811, 20 179 004]
- Training Program Project of College Students' Innovation and Entrepreneurship of Liaoning Province [201710164000026]
This study aimed to investigate the effects of transient receptor potential vanilloid 4 (TRPV4) inhibition on blood-brain barrier (BBB) integrity and the expressions of caveolae structural proteins caveolin-1 and caveolin-2 in rats with focal cerebral ischemia and reperfusion. BBB permeability was assessed by Evans blue extravasation. The mRNA and protein expressions of caveolin-1 and caveolin-2 were determined by RT-PCR, Western blot and immunohistochemistry assays. We found that BBB permeability significantly increased and reaches its peak at 72h of reperfusion in cerebral ischemia-reperfusion rats and is able to be ameliorated by administration of HC-067047, an antagonist of TRPV4. Additionally, it shows a significant upregulation of caveolin-1 and caveolin-2 expression in cerebral microvessels of ischemic tissue. However, treatment with HC-067047 was shown to downregulate caveolin-1 and caveolin-2 expression during cerebral ischemia-reperfusion. This study demonstrates that inhibition of TRPV4 ameliorates BBB leakage induced by ischemia-reperfusion injury through the downregulation of caveolin-1 and caveolin-2.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据