期刊
3RD INTERNATIONAL CONFERENCE ON TISSUE ENGINEERING (ICTE2013)
卷 59, 期 -, 页码 166-173出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.proeng.2013.05.107
关键词
Dendritic cell; Cellular Interactions; Cell Motility; T Lymphocytes; 3D culture systems
资金
- FAPESP [2010/18139-7
- 2009/54599-5]
- CNPq
We describe the interactions between monocyte-derived DCs, in different stages of maturation, with allogeneic T lymphocytes in a 3D system. Maturation of DCs increased their interaction time with T lymphocytes from 43 to 138 minutes. The average motility of T lymphocytes interacting or not with DCs was also affected, varying from 0.21 mu m-0.37 mu m/minute to 0.36 mu m-0.52 mu m/minute. These data indicate that this 3D Biotek (TM) scaffold enables interactions between lymphocytes and DCs at different stages of maturation and may be useful for the characterization of these interactions, the cellular subtypes and patterns of response induced. (C) 2013 The Authors. Published by Elsevier Ltd.
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