期刊
NEURAL REGENERATION RESEARCH
卷 13, 期 2, 页码 257-264出版社
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/1673-5374.226396
关键词
nerve regeneration; suspended moxibustion; middle cerebral artery occlusion; cerebral ischemia/reperfusion injury; infarct volume; apoptosis; Bcl-2; Bax; caspase-9; caspase-3; neural regeneration; traditional Chinese medical therapy
资金
- National Natural Science Foundation of China [81060305, 81660819]
- Natural Science Foundation of Jiangxi Province of China [2015BAB205068]
- Key Program for Science and Technology Cooperation Projects of Jiangxi Province of China [20161BBH80053]
- Key Project of Health Commission of Jiangxi Province of China [2014Z003]
- Natural Science Foundation of Jiangxi University of Traditional Chinese Medicine of China [2014ZR018, 2015jzzdxk024]
Heat-sensitive suspended moxibustion has a neuroprotective effect against focal cerebral ischemia/reperfusion injury, but the underlying mechanisms remain unclear. The duration of heat-sensitive suspended moxibustion (usually from 30 minutes to 1 hour) is longer than traditional suspended moxibustion (usually 15 minutes). However, the effects of 15- and 35-minute suspended moxibustion in rats with cerebral ischemia/reperfusion injury are poorly understood. In this study, we performed 15- or 35-minute suspended moxibustion at acupoint Dazhui (GV14) in an adult rat model of focal cerebral ischemia/reperfusion injury. Infarct volume was evaluated with the 2,3,5-triphenyltetrazolium chloride assay. Histopathological changes and neuronal apoptosis at the injury site were assessed by hematoxylineosin staining and terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Caspase-9 and caspase-3 expression at the injury site was detected using immunofluorescent staining. Bax and Bcl-2 expression at the injury site was assessed using western blot assay. In the 35-minute moxibustion group, infarct volume was decreased, neuronal apoptosis was reduced, caspase-9, caspase-3 and Bax expression was lower, and Bcl-2 expression was increased, compared with the 15-minute moxibustion group. Our findings show that 35-minute moxibustion has a greater anti-apoptotic effect than 15-minute moxibustion after focal cerebral ischemia/reperfusion injury.
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