4.5 Article

Accurate design of translational output by a neural network model of ribosome distribution

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NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 25, 期 7, 页码 577-+

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NATURE PUBLISHING GROUP
DOI: 10.1038/s41594-018-0080-2

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  1. National Cancer Institute of the National Institutes of Health [R21CA202960]
  2. National Institute of General Medical Sciences of the National Institutes of Health [P50GM102706]
  3. Department of Defense through the National Defense Science & Engineering Graduate Fellowship (NDSEG) Program
  4. National Institutes of Health S10 Instrumentation [OD018174]
  5. UC Berkeley flow cytometry core facilities

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Synonymous codon choice can have dramatic effects on ribosome speed and protein expression. Ribosome profiling experiments have underscored that ribosomes do not move uniformly along mRNAs. Here, we have modeled this variation in translation elongation by using a feed-forward neural network to predict the ribosome density at each codon as a function of its sequence neighborhood. Our approach revealed sequence features affecting translation elongation and characterized large technical biases in ribosome profiling. We applied our model to design synonymous variants of a fluorescent protein spanning the range of translation speeds predicted with our model. Levels of the fluorescent protein in budding yeast closely tracked the predicted translation speeds across their full range. We therefore demonstrate that our model captures information determining translation dynamics in vivo; that this information can be harnessed to design coding sequences; and that control of translation elongation alone is sufficient to produce large quantitative differences in protein output.

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