期刊
NATURE REVIEWS MICROBIOLOGY
卷 16, 期 6, 页码 333-339出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41579-018-0002-7
关键词
-
类别
资金
- Defense Threat Reduction Agency [HDTRA1-15-1-0051]
- Paul G. Allen Frontiers Group
- Wyss Institute for Biologically Inspired Engineering
- Broad Institute of MIT and Harvard
- Burroughs Wellcome Fund Career Award for Medical Scientists
Genetic manipulation of microorganisms has been crucial in understanding their biology, yet for many microbial species, robust tools for comprehensive genetic analysis were lacking until the advent of CRISPR-Cas-based gene editing techniques. In this Progress article, we discuss advances in CRISPR-based techniques for the genetic analysis of genetically intractable microorganisms, with an emphasis on mycobacteria, fungi and parasites. We discuss how CRISPR-based analyses in these organisms have enabled the discovery of novel gene functions, the investigation of genetic interaction networks and the identification of virulence factors.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据