期刊
NATURE PROTOCOLS
卷 13, 期 7, 页码 1506-1520出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41596-018-0001-1
关键词
-
资金
- National Key R&D Program of China [2017YFA0205501]
- National Natural Science Foundation of China [81722024, 81571728, 31530026]
- Key Research Program of Frontier Sciences [QYZDY-SSW-SMC013]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA09030306, XDPB0304]
- Youth Innovation Promotion Association [2014078]
- Sanming Project of Medicine in Shenzhen [SZSM201612031]
Nanozymes are nanomaterials exhibiting intrinsic enzyme-like characteristics that have increasingly attracted attention, owing to their high catalytic activity, low cost and high stability. This combination of properties has enabled a broad spectrum of applications, ranging from biological detection assays to disease diagnosis and biomedicine development. Since the intrinsic peroxidase activity of Fe3O4 nanoparticles (NPs) was first reported in 2007, >40 types of nanozymes have been reported that possess peroxidase-, oxidase-, haloperoxidase-or superoxide dismutase-like catalytic activities. Given the complex interdependence of the physicochemical properties and catalytic characteristics of nanozymes, it is important to establish a standard by which the catalytic activities and kinetics of various nanozymes can be quantitatively compared and that will benefit the development of nanozyme-based detection and diagnostic technologies. Here, we first present a protocol for measuring and defining the catalytic activity units and kinetics for peroxidase nanozymes, the most widely used type of nanozyme. In addition, we describe the detailed experimental procedures for a typical nanozyme strip-based biological detection test and demonstrate that nanozyme-based detection is repeatable and reliable when guided by the presented nanozyme catalytic standard. The catalytic activity and kinetics assays for a nanozyme can be performed within 4 h.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据