期刊
NATURE METHODS
卷 15, 期 7, 页码 515-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-018-0010-6
关键词
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资金
- Link program of the Dutch province of Limburg
- ITEA
- RVO [ITEA151003/ITEA 14001]
- Danish Council for Independent Research \ Natural Sciences [DFF - 6108-00493]
- Lundbeckfonden [R54-A5858]
- VILLUM Foundation [VKR023439]
- VILLUM Center for Bioanalytical Sciences [VKR023179]
- Interreg V EMR
- Netherlands Ministry of Economic Affairs within the EURLIPIDS project
We report a method that enables automated data-dependent acquisition of lipid tandem mass spectrometry data in parallel with a high-resolution mass spectrometry imaging experiment. The method does not increase the total image acquisition time and is combined with automatic structural assignments. This lipidome-per-pixel approach automatically identified and validated 104 unique molecular lipids and their spatial locations from rat cerebellar tissue.
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