期刊
NATURE METHODS
卷 15, 期 3, 页码 194-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.4578
关键词
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资金
- NIH Director's New Innovator Award [1DP2EB016573, R01 (EB020892)]
- US Army Research Office [W911NF-12-10594]
- Camille and Henry Dreyfus Foundation
Optical multiplexing has a large impact in photonics, the life sciences and biomedicine. However, current technology is limited by a 'multiplexing ceiling' from existing optical materials. Here we engineered a class of polyyne-based materials for optical supermultiplexing. We achieved 20 distinct Raman frequencies, as 'Carbon rainbow', through rational engineering of conjugation length, bond-selective isotope doping and end-capping substitution of polyynes. With further probe functionalization, we demonstrated ten-color organelle imaging in individual living cells with high specificity, sensitivity and photostability. Moreover, we realized optical data storage and identification by combinatorial barcoding, yielding to our knowledge the largest number of distinct spectral barcodes to date. Therefore, these polyynes hold great promise in live-cell imaging and sorting as well as in high-throughput diagnostics and screening.
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