期刊
NATURE METHODS
卷 15, 期 5, 页码 367-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.4661
关键词
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资金
- European Research Council [ERC CoG-724489]
- Deutsche Forschungs Gemeinschaft [DFG RI 2380/2]
- EMBL Interdisciplinary Postdoc Programme (EIPOD) under Marie Curie Actions COFUND
- 4D Nucleome/4DN NIH Common Fund [U01 EB021223]
- European Molecular Biology Laboratory
We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves minimal uncertainty in 3D on any microscope and is compatible with any PSF engineering approach. We used this method to image cellular structures and attained unprecedented image quality for astigmatic PSFs. The fitter compensates for most optical aberrations and makes accurate 3D super-resolution microscopy broadly accessible, even on standard microscopes without dedicated 3D optics.
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