期刊
NATURE METHODS
卷 15, 期 3, 页码 221-+出版社
NATURE RESEARCH
DOI: 10.1038/NMETH.4582
关键词
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资金
- NIH NIGMS [T32GM007223]
- NSF
- NIH [DP2 HD083992-01]
- Searle scholarship
RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, which uses oxidative-nucleophilic-aromatic substitution to convert 4-thiouridine into cytidine analogs, yielding apparent U-to-C mutations that mark new transcripts upon sequencing. TimeLapse-seq is a single-molecule approach that is adaptable to many applications and reveals RNA dynamics and induced differential expression concealed in traditional RNA-seq.
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