期刊
NATURE IMMUNOLOGY
卷 19, 期 5, 页码 487-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41590-018-0092-4
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资金
- Austrian Science Fund (FWF) [1953-B20, P25775-B2, P275941-B28, V538-B26]
- Singapore National Medical Research Council [NMRC/CBRG/0064/2014]
- Wellcome Trust [098274/Z/12/Z]
- Wellcome Trust [098274/Z/12/Z] Funding Source: Wellcome Trust
- Austrian Science Fund (FWF) [P27941] Funding Source: Austrian Science Fund (FWF)
- MRC [MC_UU_00008/4, MC_UU_12010/4] Funding Source: UKRI
T cell antigen recognition requires T cell antigen receptors (TCRs) engaging MHC-embedded antigenic peptides (pMHCs) within the contact region of a T cell with its conjugated antigen-presenting cell. Despite micromolar TCR:pMHC affinities, T cells respond to even a single antigenic pMHC, and higher-order TCRs have been postulated to maintain high antigen sensitivity and trigger signaling. We interrogated the stoichiometry of TCRs and their associated CD3 subunits on the surface of living T cells through single-molecule brightness and single-molecule coincidence analysis, photon-antibunching-based fluorescence correlation spectroscopy and Forster resonance energy transfer measurements. We found exclusively monomeric TCR-CD3 complexes driving the recognition of antigenic pMHCs, which underscores the exceptional capacity of single TCR-CD3 complexes to elicit robust intracellular signaling.
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