期刊
NATURE BIOTECHNOLOGY
卷 36, 期 7, 页码 614-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.4151
关键词
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资金
- NSF CAREER Award [MCB-1350949]
- DARPA grant [HR0011-15-C-0091]
- NIH [1DP2AI131083-01]
- DARPA Young Faculty Award [D16AP00142]
- NSF [CCF-1522074]
- Division of Computing and Communication Foundations
- Direct For Computer & Info Scie & Enginr [1522074] Funding Source: National Science Foundation
Precise control over microbial cell growth conditions could enable detection of minute phenotypic changes, which would improve our understanding of how genotypes are shaped by adaptive selection. Although automated cell-culture systems such as bioreactors offer strict control over liquid culture conditions, they often do not scale to high-throughput or require cumbersome redesign to alter growth conditions. We report the design and validation of eVOLVER, a scalable do-it-yourself (DIY) framework, which can be configured to carry out high-throughput growth experiments in molecular evolution, systems biology, and microbiology. High-throughput evolution of yeast populations grown at different densities reveals that eVOLVER can be applied to characterize adaptive niches. Growth selection on a genome-wide yeast knockout library, using temperatures varied over different timescales, finds strains sensitive to temperature changes or frequency of temperature change. Inspired by large-scale integration of electronics and microfluidics, we also demonstrate millifluidic multiplexing modules that enable multiplexed media routing, cleaning, vial-to-vial transfers and automated yeast mating.
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