期刊
NANOTOXICOLOGY
卷 12, 期 8, 页码 847-867出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/17435390.2018.1482378
关键词
Nanoplastics; sea urchin coelomocytes; protein-corona; toposome; MXR
资金
- Italian National Antarctic Research Program [PNRA 14_00090]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [234109/2014-9]
In order to assess the impact of nanoplastics on marine species, polystyrene nanoparticles (PS NPs) have been largely used as model particles. Here we studied the effects of 50nm amino-modified PS-NH2 on Mediterranean sea urchin Paracentrotus lividus immune system cells (coelomocytes) in the presence of celomic fluid (CF) and at different NP concentrations (1, 5, 10, and 25g mL(-1)) and experimental conditions (absence or presence of EDTA). PS-NH2 acquired a protein corona once incubated with CF, dominated by the toposome precursor protein (TPP). In short-term cultures, a significant concentration- and time-dependent decrease in lysosomal membrane stability and apoptotic-like nuclear alterations were observed in phagocytes upon exposure to PS-NH2 (10 and 25 mu g mL(-1)) in CF but they resulted abolished in the presence of EDTA confirming the role of TPP in triggering PS-NH2-coelomocytes interaction and toxicity. PS-NH2 did not alter MXR phenotype but the observed dose-dependent decrease in calcein accumulation suggests the ability of PS-NH2 to affect pump's efflux activity. Overall results encourage additional studies on positively charged nanoplastics, since the observed effects on sea urchin coelomocytes as well as the TPP corona formation might represent a first step for addressing their impact on sensitive marine species.
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