期刊
NANOTECHNOLOGY
卷 29, 期 26, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/1361-6528/aabca3
关键词
gold interdigitated electrode; capacitive biosensor; oriented antibody immobilization; hepatitis B surface antigen; immunosensor
资金
- University of Tehran
Immunosensors based on interdigitated electrodes (IDEs), have recently demonstrated significant improvements in the sensitivity of capacitance detection. Herein, a novel type of highly sensitive, compact and portable immunosensor based on a gold interdigital capacitor has been designed and developed for the rapid detection of hepatitis B surface antigen (HBsAg). To improve the efficiency of antibody immobilization and time-saving, a self-assembled monolayer (SAM) of 2-mercaptoethylamine film was coated on IDEs. Afterwards, carboxyl groups on primary antibodies were activated through 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and were immobilized on amino-terminated SAM for better control of the oriented immobilization of antibodies on gold IDEs. In addition, gold nanoparticles conjugated with a secondary antibody were used to enhance the sensitivity. Under optimal conditions, the immunosensor exhibited the sensitivity of 0.22 nF.pg ml(-1), the linear range from 5 pg ml(-1) to 1 ng ml(-1) and the detection limit of 1.34 pg ml(-1), at a signal-to-noise ratio of 3.
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