期刊
NANOMEDICINE
卷 13, 期 4, 页码 373-390出版社
FUTURE MEDICINE LTD
DOI: 10.2217/nnm-2017-0285
关键词
biosynthesis; silver nanoparticles; Leishmania
资金
- Institute Evandro Chagas, Department of Health Surveillance, Ministry of Health
- Laboratory of Electron Microscopy
- Laboratory of Superficial and Systemic Mycoses
- Laboratory of Leishmaniasis
- National Council for Scientific and Technological Development (CNPq)
- Pro-reitoria de Pesquisa da Universidade Federal do Para (PROPESP-UFPA)
- Fundacao de Amparo e Desenvolvimento da Pesquisa (FADESP)
Aim: Development of functionalized nanocomposites containing AgNPs-PVP-Glucantime (R) to evaluate their leishmanicidal activity as a novel method for improving the pharmacological properties of the drug Glucantime (R) against extracellular promastigotes and intracellular amastigotes of Leishmania amazonensis in vitro to treat cutaneous leishmaniasis. Materials & methods: The silver nanoparticles and nanocomposites prepared containing silver nanoparticles, polyvinylpyrrolidone and different amounts of Glucantime were characterized using transmission electron microscopy, x-ray diffraction, energy-dispersive x-ray spectroscopy and zeta potential analysis; in addition, the in vitro cytotoxicity was evaluated. Results: The nanocomposites showed an inhibitory effect on the cellular viability of promastigote forms, with values of 47.06, 51.71 and 65.67% for nanocomposite(1), nanocomposite(2) and nanocomposite(3), respectively, as well as a dose-dependent decrease in the infectivity index, with values of 33.33 and 23% for nanocomposite(2) and nanocomposite(3), respectively. Conclusion: The proposed nanocomposite reveals leishmanial activity and the absence of cytotoxicity in macrophages. Further investigations will be conducted in vivo.
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