期刊
NANO RESEARCH
卷 11, 期 10, 页码 5596-5603出版社
TSINGHUA UNIV PRESS
DOI: 10.1007/s12274-018-2082-0
关键词
amino-ester nanomaterials; lipid-like nanoparticles (LLNs); dual-functional; superparamagnetic iron oxide nanoparticles (SPIONs); mRNA delivery; magnetic resonance imaging (MRI)
类别
资金
- Early Career Investigator Award from the Bayer Hemophilia Awards Program
- Research Awards from the National PKU Alliance
- New Investigator Grant from the American Association of Pharmaceutical Scientists (AAPS) Foundation
- Maximizing Investigators' Research Award from the National Institute of General Medical Sciences [1R35GM119679]
- College of Pharmacy at The Ohio State University
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R35GM119679] Funding Source: NIH RePORTER
Nanoparticles have been widely explored for combined therapeutic and diagnostic applications. For example, lipid-based nanoparticles have been used to encapsulate multiple types of agents and achieve multi-functions. Herein, we enabled a co-delivery of mRNA molecules and superparamagnetic iron oxide nanoparticles (SPIONs) by using an amino-ester lipid-like nanomaterial. An orthogonal experimental design was used to identify the optimal formulation. The optimal formulation, MPA-Ab-8 LLNs, not only showed high encapsulation of both mRNA and SPIONs, but also increased the r(2) relaxivity of SPIONs by more than 1.5-fold in vitro. MPA-Ab-8 LLNs effectively delivered mRNA and SPIONs into cells, and consequently induced high protein expression as well as strong MRI contrast. Consistent herewith, we observed both mRNA-mediated protein expression and an evident negative contrast enhancement of MRI signal in mice. In conclusion, amino-ester nanomaterials demonstrate great potential as delivery vehicles for theranostic applications.
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