期刊
NANO LETTERS
卷 18, 期 4, 页码 2733-2737出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.8b00854
关键词
AFM; DNA; hydration; single-molecule biophysics
类别
资金
- Israeli Science Foundation [547/17]
- single-molecule ICore center of excellence [1902/12]
Hydration interaction shapes biomolecules and is a dominant intermolecular force. Mapping the hydration patterns of biomolecules is therefore essential for understanding molecular processes in biology. Numerous studies have been devoted to this challenge, but current methods cannot map the hydration of single biomolecules, let alone do so under physiological conditions. Here, we show that frequency-modulation atomic force microscopy (FM-AFM) can fill this gap and generate 3D hydration maps of single DNA molecules under near-physiological conditions. Additionally, we present real-space images of DNA in which the double helix is resolved with unprecedented resolution, dearly revealing individual phosphate groups along the DNA backbone. FM-AFM therefore emerges as a powerful enabling tool in the study of individual biomolecules under physiological conditions.
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