期刊
MOLECULAR SYSTEMS BIOLOGY
卷 14, 期 4, 页码 -出版社
WILEY
DOI: 10.15252/msb.20178046
关键词
cell fate; development; differentiation; single-cell RNA-seq; transcriptome
资金
- Wellcome Trust Grant Systematic Identification of Lineage Specification in Murine Gastrulation [109081/Z/15/A]
- Wellcome Trust Grant Tracing early mammalian lineage decisions by single cell genomics [105031/B/14/Z]
- Cancer Research UK [A17197]
- EMBL
- Wellcome Trust [105031/B/14/Z, 109081/Z/15/A] Funding Source: Wellcome Trust
High-throughput -omics techniques have revolutionised biology, allowing for thorough and unbiased characterisation of the molecular states of biological systems. However, cellular decision-making is inherently a unicellular process to which bulk -omics techniques are poorly suited, as they capture ensemble averages of cell states. Recently developed single-cell methods bridge this gap, allowing high-throughput molecular surveys of individual cells. In this review, we cover core concepts of analysis of single-cell gene expression data and highlight areas of developmental biology where single-cell techniques have made important contributions. These include understanding of cell-to-cell heterogeneity, the tracing of differentiation pathways, quantification of gene expression from specific alleles, and the future directions of cell lineage tracing and spatial gene expression analysis.
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