期刊
ACS APPLIED MATERIALS & INTERFACES
卷 7, 期 12, 页码 7033-7040出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b01191
关键词
DNA functionalization; gold nanoparticles; surface passivation; oligo ethylene glycol; salt-stability; specificity; variable DNA density
资金
- FIRB grant Nano-technological approaches toward tumor theragnostic
- FIRB grant Nano-solar: Photocatalytic Nanosystems for artificial photosynthesis and hydrogen production by solar-driven water splitting
We report a novel and multifaceted approach for the quick synthesis of highly stable single-stranded DNA (ssDNA) functionalized gold nanoparticles (AuNPs). The method is based on the combined effect of surface passivation by (1-mercaptoundec-11-yl)hexa(ethylene glycol) and low pH conditions, does not require any salt pretreatment or high excess of ssDNA, and can be generalized for oligonucleotides of any length or base sequence. The synthesized ssDNA-coated AuNPs conjugates are stable at salt concentrations as high as 3.0 M, and also functional and specific toward DNA-DNA hybridization, as shown from UV-vis spectrophotometry, scanning electron microscopy, gel electrophoresis, fluorescence, and small angle X-ray scattering based analyses. The method is highly flexible and shows an additional advantage of creating ssDNA-AuNP conjugates with a predefined number of ssDNA strands per particle. Its simplicity and tenability make it widely applicable to diverse biosensing applications involving ssDNA functionalized AuNPs.
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