期刊
MOLECULAR ONCOLOGY
卷 12, 期 5, 页码 659-676出版社
WILEY
DOI: 10.1002/1878-0261.12189
关键词
bone marrow-derived mesenchymal stem cells; cytokines; osteosarcoma; transendothelial migration; tumour plasticity
类别
资金
- Associazione Italiana Ricerca sul Cancro (AIRC) [8797]
- Azienda Ospedaliero Universitaria Meyer
- FIRC (Fondazione Italiana per la Ricerca sul Cancro) Fellowship
There is growing evidence to suggest that bone marrow-derived mesenchymal stem cells (BM-MSCs) are key players in tumour stroma. Here, we investigated the cross-talk between BM-MSCs and osteosarcoma (OS) cells. We revealed a strong tropism of BM-MSCs towards these tumour cells and identified monocyte chemoattractant protein (MCP)-1, growth-regulated oncogene (GRO)-alpha and transforming growth factor (TGF)-1 as pivotal factors for BM-MSC chemotaxis. Once in contact with OS cells, BM-MSCs trans-differentiate into cancer-associated fibroblasts, further increasing MCP-1, GRO-alpha, interleukin (IL)-6 and IL-8 levels in the tumour microenvironment. These cytokines promote mesenchymal to amoeboid transition (MAT), driven by activation of the small GTPase RhoA, in OS cells, as illustrated by the in vitro assay and live imaging. The outcome is a significant increase of aggressiveness in OS cells in terms of motility, invasiveness and transendothelial migration. In keeping with their enhanced transendothelial migration abilities, OS cells stimulated by BM-MSCs also sustain migration, invasion and formation of the in vitro capillary network of endothelial cells. Thus, BM-MSC recruitment to the OS site and the consequent cytokine-induced MAT are crucial events in OS malignancy.
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