期刊
MOLECULAR NUTRITION & FOOD RESEARCH
卷 62, 期 8, 页码 -出版社
WILEY
DOI: 10.1002/mnfr.201700738
关键词
adiponectin; microbiome; nonalcoholic fatty liver disease; sirtuin 1; tomatoes
资金
- USDA/NIFA [AFRI 2015-67017-23141]
- USDA/ARS [58-1950-51000-074S]
Scope: Beta-carotene-15,15'-oxygenase (BCO1) and beta-carotene-9',10'-oxygenase (BCO2) metabolize lycopene to biologically active metabolites, which can ameliorate nonalcoholic fatty liver disease (NAFLD). We investigate the effects of tomato powder (TP containing substantial lycopene (2.3 mg/g)) on NAFLD development and gut microbiome in the absence of both BCO1 and BCO2 in mice. Method and results: BCO1(-/-)/BCO2(-/-) double knockout mice were fed a high fat diet (HFD) alone (n = 9) or with TP feeding (n = 9) for 24 weeks. TP feeding significantly reduced pathological severity of steatosis and hepatic triglyceride levels in BCO1(-/-)/BCO2(-/-) mice (p < 0.04 vs HFD alone). This was associated with increased SIRT1 activity, nicotinamide phosphoribosyltransferase expression and AMP-activated protein kinase phosphorylation, and subsequently decreased lipogenesis, hepatic fatty acid uptake, and increasing fatty acid beta-oxidation (p < 0.05). TP feeding significantly decreased mRNA expression of proinflammatory genes (tnf-alpha, il-1 beta, and il-6) in both liver and mesenteric adipose tissue, which were associated with increased plasma adiponectin and hepatic adiponectin receptor-2. Multiplexed 16S rRNA gene sequencing was performed using DNA extracted from cecum fecal samples. TP feeding increased microbial richness and decreased relative abundance of the genus Clostridium. Conclusion: Dietary TP can inhibit NAFLD independent of carotenoid cleavage enzymes, potentially through increasing SIRT1 activity and adiponectin production and decreasing Clostridium abundance.
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