期刊
MOLECULAR NEUROBIOLOGY
卷 55, 期 11, 页码 8509-8521出版社
SPRINGER
DOI: 10.1007/s12035-018-0984-1
关键词
Amygdala; Tonic inhibition; GABA; Norepinephrine; Nicotine; 5HT
资金
- Deutsche Forschungsgemeinschaft [SFB 779]
- EU Joint Programme-Neurodegenerative Disease Research (JPND) project CIRCPROT
- BMBF
- EU Horizon 2020 [643417]
Tonic inhibition mediated by ambient levels of GABA that activate extrasynaptic GABA(A) receptors emerges as an essential factor that tunes neuronal network excitability in vitro and shapes behavioral responses in vivo. To address the role of neuromodulatory transmitter systems on this type of inhibition, we employed patch clamp recordings in mouse amygdala slice preparations. Our results show that the current amplitude of tonic inhibition (I-tonic) in projection neurons of the basal amygdala (BA) is increased by preincubation with the neurosteroid THDOC, while the benzodiazepine diazepam is ineffective. This suggests involvement of THDOC sensitive subunit containing GABA(A) receptors in mediating tonic inhibition. Moreover, we provide evidence that the neuromodulatory transmitters NE, 5HT, and ACh strongly enhance spontaneous IPSCs as well as I-tonic in the BA. As the increase in frequency, amplitude, and charge of sIPSCs by these neuromodulatory transmitters strongly correlated with the amplitude of I-tonic, we conclude that spill-over of synaptic GABA leads to activation of I-tonic and thereby to dampening of amygdala excitability. Since local injection of THDOC, as a positive modulator of tonic inhibition, into the BA interfered with the expression of contextual fear memory, our results point to a prominent role of I-tonic in fear learning.
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