期刊
MOLECULAR MEDICINE REPORTS
卷 17, 期 4, 页码 5088-5094出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2018.8493
关键词
cervical cancer; baicalein; NF-B; cell cycle; apoptosis
资金
- Science and Technology Department of Sichuan province [14JC01353-LH67, 2015LZCYD-S02(1/11)]
- Education Department of Sichuan Province [16ZB0195]
- Science and Technology Department of Luzhou City [2014-S-35]
To investigate the mechanism of baicalein in inducing human cervical cancer cell line C33A apoptosis. Baicalein (200 mu M) was used to treat C33A cells. Cell proliferation was tested by the MTT assay. Cell apoptosis was detected by the TUNEL assay and caspase-3 activity measurement. Cell cycle was determined by flow cytometry and associated gene expression at mRNA and protein levels. Nuclear factor (NF)-B activity was assessed by luciferase assay and western blotting. Baicalein suppressed cervical cancer cell C33A proliferation and induced cell apoptosis by activating caspase-3 activity. Baicalein blocked cell cycle in G(0)/G(1) phase through regulating the expression of associated genes. Baicalein inhibited NF-B activity by repressing nuclear translocation. Baicalein suppressed C33A proliferation and promoted cellular apoptosis by inhibiting NF-B signaling pathway. In conclusion, the results indicate that baicalein can inhibit cervical cancer cell proliferation and promote cell apoptosis by affecting NF-B activity.
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