4.5 Article

Phospholipase A1-based cross-reactivity among venoms of clinically relevant Hymenoptera from Neotropical and temperate regions

期刊

MOLECULAR IMMUNOLOGY
卷 93, 期 -, 页码 87-93

出版社

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.molimm.2017.11.007

关键词

Insect venom allergy; Phospholipase A1; Cross-reactivity; Sensitization; Molecular diagnosis

资金

  1. FAPESP (Sao Paulo Research Foundation) [2006/54799-6, 2014/13936-7, 2011/51684-1]
  2. FUNDUNESP-UNESP [01197/10-DFP]
  3. CAPES-DS (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
  4. Postgraduate Program of Biological Sciences (Cellular and Molecular Biology) at UNESP, Rio Claro
  5. CAPES-PDSE [88881.132448/2016-01]
  6. FAPESP [2013/26451-9]
  7. Council of Technological and Scientific Development (CNPq) Brazil [455422/2014-1]

向作者/读者索取更多资源

Molecular cross-reactivity caused by allergen homology or cross-reactive carbohydrate determinants (CCDs) is a major challenge for diagnosis and immunotherapy of insect venom allergy. Venom phospholipases A1 (PLA1s) are classical, mostly non-glycosylated wasp and ant allergens that provide diagnostic benefit for differentiation of genuine sensitizations from cross-reactivity. As CCD-free molecules, venom PLA1s are not causative for CCD-based cross-reactivity. Little is known however about the protein-based cross-reactivity of PLA1 within vespid species. Here, we address PLA1-based cross-reactivity among ten clinically relevant Hymenoptera venoms from Neotropical and temperate regions including Polybia paulista (paulistinha) venom and Vespula vulgaris (yellow jacket) venom. In order to evaluate cross-reactivity, sera of mice sensitized with recombinant PLA1 (rPoly p 1) from P. paulista wasp venom were used. Pronounced IgE and IgG based cross-reactivity was detected for wasp venoms regardless the geographical region of origin. The cross-reactivity correlated well with the identity of the primary sequence and 3-D models of PLA1 proteins. In contrast, these mice sera showed no reaction with honeybee (HBV) and fire ant venom. Furthermore, sera from patients monosensitized to HBV and fire ants did not recognize the rPoly p 1 in immunoblotting. Our findings reveal the presence of conserved epitopes in the PLA1s from several clinically relevant wasps as major cause of PLA1-based in vitro cross-reactivity. These findings emphasize the limitations but also the potential of PLA1-based HVA diagnostics.

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