期刊
MOLECULAR ECOLOGY
卷 27, 期 2, 页码 459-475出版社
WILEY
DOI: 10.1111/mec.14457
关键词
20-hydroxyecdysone; baculovirus; climbing behaviour; Helicoverpa armigera; juvenile hormone; miRNAs
资金
- China Agriculture Research System [CARS-28-17]
- National Natural Science Foundation of China [31572018]
Baculoviruses manipulate host climbing behaviour to ensure that the hosts die at elevated positions on host plants to facilitate virus proliferation and transmission, which is a process referred to as tree-top disease. However, the detailed molecular mechanism underlying tree-top disease has not been elucidated. Using transcriptome analysis, we showed that two hormone signals, juvenile hormone (JH) and 20-hydroxyecdysone (20E), are key components involved in HaSNPV-induced tree-top disease in Helicoverpa armigera larvae. RNAi-mediated knockdown and exogenous hormone treatment assays demonstrated that 20E inhibits virus-induced tree-top disease, while JH mediates tree-top disease behaviour. Knockdown of BrZ2, a downstream signal of JH and 20E, promoted HaSNPV-induced tree-top disease. We also found that two miRNAs target BrZ2 and are involved in the cross-talk regulation between 20E and JH manipulating HaSNPV replication, time to death and HaSNPV-induced tree-top disease.
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