期刊
MOLECULAR CELL
卷 70, 期 6, 页码 1111-+出版社
CELL PRESS
DOI: 10.1016/j.molcel.2018.05.021
关键词
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资金
- Wellcome Trust
- MRC
- BBSRC
- BBSRC [BB/N007816]
- BBSRC DTP studentship
- BBSRC [BB/H012249/1, BB/N007816/1] Funding Source: UKRI
- MRC [MR/N006828/1] Funding Source: UKRI
Gene transcription is carried out by multi-subunit RNA polymerases (RNAPs). Transcription initiation is a dynamic multi-step process that involves the opening of the double-stranded DNA to form a transcription bubble and delivery of the template strand deep into the RNAP for RNA synthesis. Applying cryoelectron microscopy to a unique transcription system using s sigma(54) (sigma(N)), the major bacterial variant sigma factor, we capture a new intermediate state at 4.1 angstrom where promoter DNA is caught at the entrance of the RNAP cleft. Combining with new structures of the open promoter complex and an initial de novo transcribing complex at 3.4 and 3.7 angstrom, respectively, our studies reveal the dynamics of DNA loading and mechanism of transcription bubble stabilization that involves coordinated, large-scale conformational changes of the universally conserved features within RNAP and DNA. In addition, our studies reveal a novel mechanism of strand separation by sigma(54).
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