期刊
MOLECULAR CANCER
卷 17, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12943-018-0761-9
关键词
LncRNAs; Pancreatic cancer; Invasion; Epithelial mesenchymal transition; NLRP3
资金
- National Natural Science Foundation of China [81272382, 81672449]
- Social Development of Science and Technology Research Projects of Jiangsu Province [BE2016788]
- Blue Project of Colleges and Universities of Jiangsu Province [2012-16]
- Priority Academic Program Development of Jiangsu Higher Education Institutions [JX10231801]
- Innovation Capability Development Project of Jiangsu Province [BM2015004]
- Jiangsu Key Medical Discipline (General Surgery) [ZDXKA2016005]
- Special Funding of Science and Education Enhancing Health of Wuxi [QNRC015]
- Young Researcher Grants Program of Wuxi Health and Family Planning Commission [Q201621]
- Science and Technology Bureau guidance project of Wuxi [CSZ0N1709]
- Medical Core Member Science Fund of The Third Hospital Affiliated to Nantong University [2016-2018-10221]
Background: Long non-coding RNAs (lncRNAs) play an important role in the development and progression of various tumors, including pancreatic cancer (PC). Recent studies have shown that lncRNAs can 'act in cis' to regulate the expression of its neighboring genes. Previously, we used lncRNAs microarray to identify a novel lncRNA termed XLOC_000647 that was down-regulated in PC tissues. However, the expression and function of XLOC_000647 in PC remain unclear. Methods: The expression of XLOC_000647 and NLRP3 in PC specimens and cell lines were detected by quantitative real-time PCR. Transwell assays were used to determine migration and invasion of PC cells. Western blot was carried out for detection of epithelial-mesenchymal transition (EMT) markers in PC cells. The effect of XLOC_000647 on PC cells was assessed in vitro and in vivo. The function of NOD-like receptor family pyrin domain-containing 3 (NLRP3) in PC was investigated in vitro. In addition, the regulation of NLRP3 by XLOC_000647 in PC was examined in vitro. Results: Here, XLOC_000647 expression was down-regulated in PC tissues and cell lines. The expression level of XLOC_000647 was significantly correlated to tumor stage, lymph node metastasis, and overall survival. Overexpression of XLOC_000647 attenuated cell proliferation, invasion, and EMT in vitro and impaired tumor growth in vivo. Further, a significantly negative correlation was observed between XLOC_000647 levels and its genomic nearby gene NLRP3 in vitro and in vivo. Moreover, XLOC_000647 decreased NLRP3 by inhibiting its promoter activity. Knockdown of NLRP3 decreased proliferation of cancer cells, invasion, and EMT in vitro. Importantly, after XLOC_000647 was overexpressed, the corresponding phenotypes of cells invasion and EMT were reversed by overexpression of NLRP3. Conclusions: Together, these results indicate that XLOC_000647 functions as a novel tumor suppressor of lncRNA and acts as an important regulator of NLRP3, inhibiting cell proliferation, invasion, and EMT in PC.
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