期刊
MOLECULAR BIOTECHNOLOGY
卷 60, 期 6, 页码 396-411出版社
HUMANA PRESS INC
DOI: 10.1007/s12033-018-0079-2
关键词
Cell differentiation; Endothelial-like cells; Hypoxic environment; Mesenchymal stem cell; Smooth muscle cells
资金
- National Natural Science Foundation of China [81671407]
Endothelial cells and smooth muscle cells (SMCs) are important aspects of vascularization in vaginal reconstruction. Research has confirmed that mesenchymal stem cells could differentiate into endothelial-like cells and SMCs. But the methods were more complicated and the mechanism was unknown. In the current study, we induced the bone mesenchymal stem cells (BMSCs) to differentiate into endothelial-like cells and SMCs in vitro by differentiation medium and investigated the effect of Wnt/beta-catenin signaling on the differentiation process of BMSCs. Results showed that the hypoxic environment combined with VEGF and bFGF could induce increased expression of endothelial-like cells markers VEGFR1, VEGFR2, and vWF. The SMCs derived from BMSCs induced by TGF-beta 1 and PDGF-AB significantly expressed SMC markers SMMHC11 and alpha-SMA. The data also showed that activation of Wnt/beta-catenin signaling could promote the differentiation of BMSCs into endothelial-like cells and SMCs. Thus, we established endothelial-like cells and SMCs in vitro by more simple methods, presented the important role of hypoxic environment on the differentiation of BMSCs into endothelial-like cells, and confirmed that the Wnt/beta-catenin signaling pathway has a positive impact on the differentiation of BMSCs into endothelial-like cells and SMCs. This is important for vascular reconstruction.
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