期刊
MOLECULAR BIOLOGY REPORTS
卷 45, 期 5, 页码 1545-1550出版社
SPRINGER
DOI: 10.1007/s11033-018-4241-5
关键词
Mediator complex; Transcription; Adipogenesis; Human mesenchymal stem cells
资金
- Center for Cardiovascular Diseases and Sciences
- Center for Molecular and Tumor Virology
- Feist-Weiller Cancer Center
- Louisiana Biomedical Research Network, an NIH INBRE grant [8P20GM103424]
- Louisiana Board of Regents Pilot Funding Program
- Louisiana Tech University College of Applied and Natural Sciences
- Louisiana Tech University College of School of Biological Sciences
Regulation of gene expression is critical for the maintenance of cell state and homeostasis. Aberrant regulation of genes can lead to unwanted cell proliferation or misdirected differentiation. Here we investigate the role of MED31, a highly conserved subunit of the Mediator complex, to determine the role this subunit plays in the maintenance of human mesenchymal stem cell (hMSC) state. Using siRNA-mediated knockdown of MED31 we demonstrate a decrease in self-renewal based on cell assays and monitoring of gene expression. In addition, in the absence of MED31, hMSCs also displayed a reduction in adipogenesis as evidenced by diminished lipid vesicle formation and expression of specific adipogenic markers. These data present evidence for a significant role for MED31 in maintaining adult stem cell homeostasis, thereby introducing potential novel targets for future investigation and use in better understanding stem cell behavior and adipogenesis.
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